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We use cookies to offer you a better browsing experience, analyze site traffic, personalize content, and serve targeted advertisements. Read about how we use cookies and how you can control them in our Privacy Policy. If you continue to use this site, you consent to our use of cookies.Cell-based activity is perhaps best exemplified by cytokines, such as IL-2, which directly and specifically acts upon cell surface receptors. This activity of a cytokine is often mediated through a specific receptor on the surface of the cells to be affected. IL-2 binds to a specific receptor on the surface of T cells, and this binding causes IL-2 to be internalized into the T cell. By secreting cytokine, as IL-2 does, it is able to directly affect the function of the T cell.
The IL-2 receptor complex is a non-covalently associated heterodimer. The subunits of this receptor complex are composed of two distinct receptors, IL-2 receptor-.alpha. and IL-2 receptor-.beta. The major function of IL-2 receptor is to mediate the response of T cells to IL-2. As a result, the IL-2 receptor is also known as the CD25 receptor. Recent evidence indicates that T cells that overexpress the IL-2 receptor may be characterized by a more activated state of immune activity. For example, active T cells that are found in response to an infection or autoimmune disease, may be characterized as having high levels of the IL-2 receptor.
As a direct result of the increased expression of the IL-2 receptor on T cells, there is now a large effort to develop small molecules as therapeutics to specifically inhibit the response of T cells. The molecular approach of designing small molecules to block protein-protein interactions has been widely explored. It is in part because of the rapid progress made by the biotechnology industry in the characterization of the fundamental components of various protein-protein interactions (see, e.g. Coulier et al., Chapter 25, Imaging Proteins: Biophysics and Biology in the New Millenium, Academic Press, 1998) that lead to the discovery of small-molecule therapeutic agents.
Chimeric and humanized antibodies have been targeted to cell surface receptors to inhibit the responses of T cells. Antibodies that bind to the surface receptors can be designed to not only target the IL-2 receptor complex, but to the subunits thereof. The application of antibody-based therape
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